Topical bioformulation for wound healing
Therapeutic based biopreparations for
treatment of all kinds of acute and chronic wounds
We are working with proprietary recombinant protein
bio-formulations which demonstrates extremely high potency
for wound regeneration. Unlike already known technologies, our
prototype wound healing compositions do not comprise growth
factors or proteases.
Efficiently combined biologically active recombinant proteins with
topical pharmaceutical formulations are aimed for acceleration of
wound healing process and thus should enable to reduce risks of
complications and or increase quality of life.
Two prototypes of topical bio-formulations are in the scope:
• Wound Healing Preparation 1
• Wound Healing Preparation 2
Both Prep1 and Prep2 bio-formulations demonstrated significant
wound healing effects for during trials with in vitro and in vivo
models, thus we are considering them as potential candidates for
clinical trials. Patent application for Prep1 and Prep2 was filed.
Stability testing of Prep1 and Prep2
Accelerated stability studies of the current Prep1 and Prep2
bio-formulations were successfully conducted using functional
wound healing assay-in vitro test, which is based on cell culture
monolayer scratch and recovery evaluation (see example in
Panel 1). Cell culture monolayer was formed by epidermis:
HaCAT-KER-CTh and dermis: TERT and BJ-5ta, hTERT, cell
lines.
Prep1 and Prep2 showed full functional activity sustaining
after incubation of both prototypes at 23 and 37 °C. Real-time
stability studies of Prep1 and Prep2 are still on-going.
Conclusion and future perspectives
Herein, we investigated the treatment feasibility of acute/surgical
wounds. Results of our investigation, showed that both, Prep1
and Perp2 bio-formulations had a significantly positive impact on
acceleration of wound healing of mice models.
Next steps
• Evaluate the potential of bio-formulations for treatment of
difficult healing chronic wounds related to diabetes, skin
inflammation, immunodeficiency and burn trauma.
• Focus on the new developments and formulations for
treatment of broad spectrum of eye injuries.
Wound Healing Preparation 1 and 2 (Prep1 and Prep2)—in vitro model
Prep1 wound healing scratch test:
C1—Layer of human epithelium cells after scratch
C2—Layer of human epithelium cells after scratch and
constant treatment with hydrogen peroxide
Prep1—Layer of human epithelium cells after scratch,
constant treatment with hydrogen peroxide in combination
with treatment of “Wound Healing Preparation 1”
Prep2—Layer of human epithelium cells after scratch,
constant treatment with hydrogen peroxide in combination
with treatment of “Wound Healing Preparation 2”
The results revealed that restoration of cell layer was
achieved significantly faster due to treatment with
Prep1 and Prep2 formulations in both experimental
set-ups after scratch of human epithelium cells layer
with and without hydrogen peroxide exposure.
Wound Healing Preparation 1 and 2 (Prep1 and Prep2)—in vivo model
Panel 2. Images of wound healing progress of investigated mice groups
after excision injury. Control group was not subjected to bio-formulations,
while P1 and P2 groups were treated with bio-formulation Prep1 and Prep2,
respectively. The images were randomly selected from the group individuals.
Chart 1. Wound are change during 14 day course. 100% wound area represents
zero point within all mice groups after excision injury. Control group (C) was not
subjected to treatmet, while P1 and P2 groups were treated with bio-formulations
(Prep1) and 2 (Prep2), respectively. SD values were ranging within the range of 5-15%.
Wound healing evalution in vivo using mice strain C57BL/6J:
• Three groups of mice were used for systematic
evaluation of wound healing effects of two bioformulations.
• Mice groups: Control (C) without treatment, P1 and
P2 after injurie treatment with bio-formulations Prep1
and Prep2. The injurie was created by excision as
described by Fisher KS et al. 2023; and Wang X et al.
2013.
• The treatment using Prep 1 and Prep 2 was continued
and monitored for 14 days. The healing effects were
evaluated by measuring wound area with ImageJ
(Fiji). The percentage of wound area change during
the healing course was used for generation of healing
charts. 100 % value represents area of the fresh wound
at the healing starting point. Paraffin-embedded (FFPE)
tissue samples were collected after the treatment
course with Prep1 and Prep2, and Control group,
respectively.
Experimental data revealed: reduction of wound area
was achieved significantly faster in the mice groups of
P1 and P2 which were treated with Prep1 and PreP2,
respectively.
Contact us at info@bioremedium.com
Universiteto g. 3, LT-01131 Vilnius
